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10X Genomics chromium single cell b chip kit
Chromium Single Cell B Chip Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chromium+single+cell+b+chip+kit/cellranger/pmc13198283-109-0-7
Average 86 stars, based on 1 article reviews
chromium single cell b chip kit - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling

Reverse Transcription:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling

Purification:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling

Single Cell:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling

Article Title: A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade glioma
Article Snippet: Chromium Next GEM Single Cell Multiome ATAC + GEX Kit , 10x Genomics , Cat# PN-1000283. .. Chromium Single Cell B Chip Kit , 10x Genomics , Cat# PN-1000073. .. PhenoCycler-Fusion Instrument , Akoya Biosciences , Cat# AB-000011.

Article Title: The local microenvironment suppresses the synergy between irradiation and anti-PD1 therapy in breast-to-brain metastasis.
Article Snippet: Compound Sakura Finetek Cat# 45833 Donkey Serum Sigma Aldrich Cat#S30-M Fluorescence Mounting Medium Dako Cat#S302380-2 Pentobarbital CHUV N/A D(+)-Sucrose for molecular biology PanReac AppliChem Cat#A2211,1000 DMEM/F-12 Gibco, ThermoFisher Cat#11320033 Penicillin-Streptomycin (10,000 U/mL) Gibco, ThermoFisher, Cat# 15070063 Gadovist (Gadobutrol) Bayer N/A UltraPureTM 0.5M EDTA Invitrogen, ThermoFisher Cat#15575020 RBC Lysis Buffer (10X) BioLegend Cat#420301 Trypan Blue Solution, 0.4% Gibco, ThermoFisher Cat#15250061 Brilliant Stain Buffer BD Biosciences Cat#563794 Trypsin-EDTA (0.05%), phenol red Gibco, ThermoFisher Cat#25300062 AttaneTM Isoflurane Attane N/A Buprenorphine, Tamgesic 0.3 mg/ml Eumedica Pharmaceuticals AG Cat:#N02AE01 Bupivacaine Carbostesin, Aspen Pharma Schweiz Cat:#N01BB01 Lidocain Streuli 2%Inj Streuli Pharma AG Cat:#N01BB02 70% Ethanol pads Fisher Healthcare Cat:#502522446 10% Betadine pads Purdue Products L.P. N/A HBSS, calcium, magnesium, no phenol red Thermo Fisher Scientific Cat:#14025092 3M Vetbond Fisher scientific Cat:#17150156 Dafalgan UPSA Switzerland Cat:#N02BE01 Matrigel Corning Cat:#356231 CellTiter-Blue Promega Cat:#G8080 DAPI Thermo Fisher Scientific Cat:#D1306 Dako mounting medium Dako Cat:#S302380 RIPA buffer Thermo Fisher Scientific Cat:#89900 Halt Protease inhibitor cocktail Thermo Fisher Scientific Cat:#87785 Pierce BCA protein assay kit Thermo Fisher Scientific Cat:#23225 RedDot 1 Far-Red Nuclear Stain Chemie Bruschwig Cat:#BIO40060-1 CellTrace far red fluorescence Thermo Fisher Scientific Cat:#C34572 b-Mercaptoethanol Thermo Fisher Scientific Cat:#21985023 HEPES Thermo Fisher Scientific Cat:#15630056 mIL2 Milteny Cat:#130-120-333 CD3/CD28 Dynabeads Thermo Fisher Scientific Cat:#11456D Dimethyl sulfoxide PanReac AppliChem Cat:#A3672 Anti-CD11b microbeads Miltenyi Cat:# 130-049-601 Critical commercial assays Tumor Dissociation Kit, mouse Miltenyi Cat#130-096-730 Direct-zol RNA Miniprep kit Zymo Research Cat:#R2050 Transcripotor Fisrt Strand cDNA Synthesis Kit Roche Cat:#-4379012001 TaqMan Real-Time PCR assays Thermo Fisher Scientific N/A Chromium Single cell 30GEM kit v3 (10x, 1000077) 10x Genomics N/A (Continued on next page) Cell Reports 44, 115427, March 25, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single cell B chip kit (10x, 1000074) 10x Genomics N/A Chromium single cell 30 library kit v3 (10x, 1000078) 10x Genomics N/A Naive CD8 T cell isolation kit (EasySep) STEMCELL technologies Cat:#19858 Anti-Ly6G microbeads Miltenyi Cat:#130-120-337 CD8 T cell isolation kit (EasySep) STEMCELL technologies Cat:#19858 Deposited data Single cell RNA-seq data This paper https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE231657 Mouse reference genome mm10 Ensembl 93 v3.1.0 (Jul 24, 2019) 10x Genomics https://www.10xgenomics.com/ support/software/cell-ranger/latest/ release-notes/cr-referencerelease-notes#3.1.0 Experimental models: Cell lines PyMT-BrM3 (breast-BrM) Croci et al.20 N/A Experimental models: Organisms/strains Mouse: C57BL/6J background Charles River N/A Oligonucleotides .. Ubc Mm01201237_m1 Thermo Fisher Scientific AG N/A

Article Title: Wnt 3a-Modified Scaffolds Improve Nerve Regeneration by Boosting Schwann Cell Function
Article Snippet: .. Using single cell 3 ’Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074), the cell suspension (300–600 living cells per microliter determined by Count Star) was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer’s protocol. ..

Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons
Article Snippet: Chromium Single Cell 3′ GEM, Library & Gel Bead Kit v3 , 10X Genomics , Cat# PN-1000092. .. Chromium Single Cell B Chip Kit , 10X Genomics , Cat# PN-1000154. .. Chromium i7 Multiplex Kit , 10X Genomics , Cat# PN-120262.

Article Title: Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
Article Snippet: Finally, nuclei were stained with trypan blue (Thermo Fisher Scientific) and counted using a dual-fluorescence cell counter (Thermo Fisher Scientific). .. The suspension of nuclei was loaded onto a Chromium Single Cell Controller (10x Genomics) to produce single-nucleus gel beads in emulsion using a Single Cell 3’ Library and Gel Bead Kit V3.1 (1000075; 10x Genomics) and Chromium Single Cell B Chip Kit (1000074; 10x Genomics), in accordance with the manufacturer’s instructions. ..

Article Title: Single-cell RNA sequencing unravels T cell exhaustion underlying the chronicity of chromoblastomycosis
Article Snippet: Single-cell suspensions were adjusted to a final concentration of 800–1000 viable cells/μL, as measured using the Countstar system, prior to loading onto the Chromium Single Cell Controller (10x Genomics). .. Libraries were generated using the Single Cell 3′ Library and Gel Bead Kit v3.1 (10x Genomics; cat. no. 1000075) together with the Chromium Single Cell B Chip Kit (10x Genomics; cat. no. 1000074), following the manufacturer’s protocol for droplet-based partitioning and formation of gel bead-in-emulsions (GEMs). ..

Software:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling

Microscopy:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling

Cell Isolation:

Article Title: The local microenvironment suppresses the synergy between irradiation and anti-PD1 therapy in breast-to-brain metastasis.
Article Snippet: Compound Sakura Finetek Cat# 45833 Donkey Serum Sigma Aldrich Cat#S30-M Fluorescence Mounting Medium Dako Cat#S302380-2 Pentobarbital CHUV N/A D(+)-Sucrose for molecular biology PanReac AppliChem Cat#A2211,1000 DMEM/F-12 Gibco, ThermoFisher Cat#11320033 Penicillin-Streptomycin (10,000 U/mL) Gibco, ThermoFisher, Cat# 15070063 Gadovist (Gadobutrol) Bayer N/A UltraPureTM 0.5M EDTA Invitrogen, ThermoFisher Cat#15575020 RBC Lysis Buffer (10X) BioLegend Cat#420301 Trypan Blue Solution, 0.4% Gibco, ThermoFisher Cat#15250061 Brilliant Stain Buffer BD Biosciences Cat#563794 Trypsin-EDTA (0.05%), phenol red Gibco, ThermoFisher Cat#25300062 AttaneTM Isoflurane Attane N/A Buprenorphine, Tamgesic 0.3 mg/ml Eumedica Pharmaceuticals AG Cat:#N02AE01 Bupivacaine Carbostesin, Aspen Pharma Schweiz Cat:#N01BB01 Lidocain Streuli 2%Inj Streuli Pharma AG Cat:#N01BB02 70% Ethanol pads Fisher Healthcare Cat:#502522446 10% Betadine pads Purdue Products L.P. N/A HBSS, calcium, magnesium, no phenol red Thermo Fisher Scientific Cat:#14025092 3M Vetbond Fisher scientific Cat:#17150156 Dafalgan UPSA Switzerland Cat:#N02BE01 Matrigel Corning Cat:#356231 CellTiter-Blue Promega Cat:#G8080 DAPI Thermo Fisher Scientific Cat:#D1306 Dako mounting medium Dako Cat:#S302380 RIPA buffer Thermo Fisher Scientific Cat:#89900 Halt Protease inhibitor cocktail Thermo Fisher Scientific Cat:#87785 Pierce BCA protein assay kit Thermo Fisher Scientific Cat:#23225 RedDot 1 Far-Red Nuclear Stain Chemie Bruschwig Cat:#BIO40060-1 CellTrace far red fluorescence Thermo Fisher Scientific Cat:#C34572 b-Mercaptoethanol Thermo Fisher Scientific Cat:#21985023 HEPES Thermo Fisher Scientific Cat:#15630056 mIL2 Milteny Cat:#130-120-333 CD3/CD28 Dynabeads Thermo Fisher Scientific Cat:#11456D Dimethyl sulfoxide PanReac AppliChem Cat:#A3672 Anti-CD11b microbeads Miltenyi Cat:# 130-049-601 Critical commercial assays Tumor Dissociation Kit, mouse Miltenyi Cat#130-096-730 Direct-zol RNA Miniprep kit Zymo Research Cat:#R2050 Transcripotor Fisrt Strand cDNA Synthesis Kit Roche Cat:#-4379012001 TaqMan Real-Time PCR assays Thermo Fisher Scientific N/A Chromium Single cell 30GEM kit v3 (10x, 1000077) 10x Genomics N/A (Continued on next page) Cell Reports 44, 115427, March 25, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single cell B chip kit (10x, 1000074) 10x Genomics N/A Chromium single cell 30 library kit v3 (10x, 1000078) 10x Genomics N/A Naive CD8 T cell isolation kit (EasySep) STEMCELL technologies Cat:#19858 Anti-Ly6G microbeads Miltenyi Cat:#130-120-337 CD8 T cell isolation kit (EasySep) STEMCELL technologies Cat:#19858 Deposited data Single cell RNA-seq data This paper https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE231657 Mouse reference genome mm10 Ensembl 93 v3.1.0 (Jul 24, 2019) 10x Genomics https://www.10xgenomics.com/ support/software/cell-ranger/latest/ release-notes/cr-referencerelease-notes#3.1.0 Experimental models: Cell lines PyMT-BrM3 (breast-BrM) Croci et al.20 N/A Experimental models: Organisms/strains Mouse: C57BL/6J background Charles River N/A Oligonucleotides .. Ubc Mm01201237_m1 Thermo Fisher Scientific AG N/A

Single Cell RNA-Seq:

Article Title: The local microenvironment suppresses the synergy between irradiation and anti-PD1 therapy in breast-to-brain metastasis.
Article Snippet: Compound Sakura Finetek Cat# 45833 Donkey Serum Sigma Aldrich Cat#S30-M Fluorescence Mounting Medium Dako Cat#S302380-2 Pentobarbital CHUV N/A D(+)-Sucrose for molecular biology PanReac AppliChem Cat#A2211,1000 DMEM/F-12 Gibco, ThermoFisher Cat#11320033 Penicillin-Streptomycin (10,000 U/mL) Gibco, ThermoFisher, Cat# 15070063 Gadovist (Gadobutrol) Bayer N/A UltraPureTM 0.5M EDTA Invitrogen, ThermoFisher Cat#15575020 RBC Lysis Buffer (10X) BioLegend Cat#420301 Trypan Blue Solution, 0.4% Gibco, ThermoFisher Cat#15250061 Brilliant Stain Buffer BD Biosciences Cat#563794 Trypsin-EDTA (0.05%), phenol red Gibco, ThermoFisher Cat#25300062 AttaneTM Isoflurane Attane N/A Buprenorphine, Tamgesic 0.3 mg/ml Eumedica Pharmaceuticals AG Cat:#N02AE01 Bupivacaine Carbostesin, Aspen Pharma Schweiz Cat:#N01BB01 Lidocain Streuli 2%Inj Streuli Pharma AG Cat:#N01BB02 70% Ethanol pads Fisher Healthcare Cat:#502522446 10% Betadine pads Purdue Products L.P. N/A HBSS, calcium, magnesium, no phenol red Thermo Fisher Scientific Cat:#14025092 3M Vetbond Fisher scientific Cat:#17150156 Dafalgan UPSA Switzerland Cat:#N02BE01 Matrigel Corning Cat:#356231 CellTiter-Blue Promega Cat:#G8080 DAPI Thermo Fisher Scientific Cat:#D1306 Dako mounting medium Dako Cat:#S302380 RIPA buffer Thermo Fisher Scientific Cat:#89900 Halt Protease inhibitor cocktail Thermo Fisher Scientific Cat:#87785 Pierce BCA protein assay kit Thermo Fisher Scientific Cat:#23225 RedDot 1 Far-Red Nuclear Stain Chemie Bruschwig Cat:#BIO40060-1 CellTrace far red fluorescence Thermo Fisher Scientific Cat:#C34572 b-Mercaptoethanol Thermo Fisher Scientific Cat:#21985023 HEPES Thermo Fisher Scientific Cat:#15630056 mIL2 Milteny Cat:#130-120-333 CD3/CD28 Dynabeads Thermo Fisher Scientific Cat:#11456D Dimethyl sulfoxide PanReac AppliChem Cat:#A3672 Anti-CD11b microbeads Miltenyi Cat:# 130-049-601 Critical commercial assays Tumor Dissociation Kit, mouse Miltenyi Cat#130-096-730 Direct-zol RNA Miniprep kit Zymo Research Cat:#R2050 Transcripotor Fisrt Strand cDNA Synthesis Kit Roche Cat:#-4379012001 TaqMan Real-Time PCR assays Thermo Fisher Scientific N/A Chromium Single cell 30GEM kit v3 (10x, 1000077) 10x Genomics N/A (Continued on next page) Cell Reports 44, 115427, March 25, 2025 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chromium Single cell B chip kit (10x, 1000074) 10x Genomics N/A Chromium single cell 30 library kit v3 (10x, 1000078) 10x Genomics N/A Naive CD8 T cell isolation kit (EasySep) STEMCELL technologies Cat:#19858 Anti-Ly6G microbeads Miltenyi Cat:#130-120-337 CD8 T cell isolation kit (EasySep) STEMCELL technologies Cat:#19858 Deposited data Single cell RNA-seq data This paper https://www.ncbi.nlm.nih.gov/ geo/query/acc.cgi?acc=GSE231657 Mouse reference genome mm10 Ensembl 93 v3.1.0 (Jul 24, 2019) 10x Genomics https://www.10xgenomics.com/ support/software/cell-ranger/latest/ release-notes/cr-referencerelease-notes#3.1.0 Experimental models: Cell lines PyMT-BrM3 (breast-BrM) Croci et al.20 N/A Experimental models: Organisms/strains Mouse: C57BL/6J background Charles River N/A Oligonucleotides .. Ubc Mm01201237_m1 Thermo Fisher Scientific AG N/A

Suspension:

Article Title: Wnt 3a-Modified Scaffolds Improve Nerve Regeneration by Boosting Schwann Cell Function
Article Snippet: .. Using single cell 3 ’Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074), the cell suspension (300–600 living cells per microliter determined by Count Star) was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer’s protocol. ..

Article Title: Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
Article Snippet: Finally, nuclei were stained with trypan blue (Thermo Fisher Scientific) and counted using a dual-fluorescence cell counter (Thermo Fisher Scientific). .. The suspension of nuclei was loaded onto a Chromium Single Cell Controller (10x Genomics) to produce single-nucleus gel beads in emulsion using a Single Cell 3’ Library and Gel Bead Kit V3.1 (1000075; 10x Genomics) and Chromium Single Cell B Chip Kit (1000074; 10x Genomics), in accordance with the manufacturer’s instructions. ..

Emulsion:

Article Title: Wnt 3a-Modified Scaffolds Improve Nerve Regeneration by Boosting Schwann Cell Function
Article Snippet: .. Using single cell 3 ’Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074), the cell suspension (300–600 living cells per microliter determined by Count Star) was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer’s protocol. ..

Article Title: Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
Article Snippet: Finally, nuclei were stained with trypan blue (Thermo Fisher Scientific) and counted using a dual-fluorescence cell counter (Thermo Fisher Scientific). .. The suspension of nuclei was loaded onto a Chromium Single Cell Controller (10x Genomics) to produce single-nucleus gel beads in emulsion using a Single Cell 3’ Library and Gel Bead Kit V3.1 (1000075; 10x Genomics) and Chromium Single Cell B Chip Kit (1000074; 10x Genomics), in accordance with the manufacturer’s instructions. ..

Generated:

Article Title: Single-cell RNA sequencing unravels T cell exhaustion underlying the chronicity of chromoblastomycosis
Article Snippet: Single-cell suspensions were adjusted to a final concentration of 800–1000 viable cells/μL, as measured using the Countstar system, prior to loading onto the Chromium Single Cell Controller (10x Genomics). .. Libraries were generated using the Single Cell 3′ Library and Gel Bead Kit v3.1 (10x Genomics; cat. no. 1000075) together with the Chromium Single Cell B Chip Kit (10x Genomics; cat. no. 1000074), following the manufacturer’s protocol for droplet-based partitioning and formation of gel bead-in-emulsions (GEMs). ..

RNA sequencing:

Article Title: Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.
Article Snippet: Article Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing noncanonical Wnt signaling



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